WebIt consists of over 4,000 authenticated and characterized cell lines and hybridomas, including various panels of cancer derived tumor lines, … WebResource savings: Single cell-derived hybridomas form visible discrete colonies in semi-solid medium, and are easy to pick and screen, and transfer to liquid medium for expansion; Cloning efficiency: Individual cells are suspended and immobilized in semi-solid medium, preventing loss of rare, high-producing clones by overgrowth

High density and scale-up cultivation of recombinant CHO cell

WebNational Center for Biotechnology Information WebHybridomas – Cell line formed by fusing two different but related cells. Immortal or Continuous cell line – Cells that have been adapted to grow indefinite in culture. ... Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Observe the cells under the microscope for detachment. If the cells are less than 90% ... too much brain power

Hybridoma technology; advancements, clinical significance, and …

WebIn this work, a recombinant p30 protein produced in an insect cell system was purified and used to immunize Balb-c mice. Six anti-p30 mAbs-secreting hybridomas clone cells were obtained. These mAbs displayed high reactivity against the recombinant protein, but only 2B8E10 mAb showed excellent functionality against the p30 protein produced by ASFV. WebRegarding the threat of mycoplasma contamination of myeloma cell lines and hybridomas maintained in our laboratory (Granatova et al. 1988), we tried to detect mycoplasms in these semi-sus-pension cultures by the fluorescence method using Vero cells as indicators. Materials and Methods Myeloma cells and hybridomas (Table 1) were cultured in an ... physiological motion