Hieff trans
Web12 de abr. de 2024 · DMEM/F-12 with no phenol red was purchased from HyClone Proteins (Logan, UT, USA). Hieff Trans Liposomal Transfection Reagent and DH5α chemically competent cells were purchased from Yeasen Biotechnology (Shanghai, China). Web1 de ago. de 2024 · Transfection with plasmid DNA was performed with Hieff trans -liposomal transfection reagent (Yeasen Biotech, China) according to the manufacturer's instructions. To obtain stably-expressing Spike protein, HEK293A cells were transiently transfected with plasmid pcDNA3.1-Spike-Myc harboring full-length SARS-CoV2 Spike …
Hieff trans
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WebProduct Name: Hieff TransTM Liposomal Transfection Reagent 2. Recommended use of the chemical and restrictions on use Relevant identified uses: For research use only. Uses advised against: Not used for diagnostic procedures. 3. Details of the supplier of the safety data sheet Yeasen Biotechnology (Shanghai) Co., Ltd. Web30 de jul. de 2024 · Using Hieff Trans™ Liposomal Transfection Reagent (Yeasen, China), H1299 cells were transiently transfected with miniTurbo-p53 plasmids. After transfection with mCherry-miniTurbo-p53 plasmids (48 h), positive cells were selected in DMEM supplemented with 700 μg/mL of geneticin (Diamond, USA) for approximately 20 days. …
Web产品描述 Hieff Trans ® 通用型转染试剂 是基于最新的纳米技术研发的一款多用途、便捷高效的脂质体转染试剂，适用于 DNA 、 RNA 和寡核苷酸的转染，对难转染细胞 也 具有较高的转染效率。 其独特的配方使其可直接加入培养基中，血清的存在不会影响转染效率，这样可以减少去除血清对细胞的损伤。 WebProduct Description. Hieff Trans™ universal transfection reagent is a multi-purpose, convenient and efficient liposome transfection reagent developed based on the latest …
Web7 de abr. de 2024 · 通过调整DNA/Hieff Trans?脂质体核酸转染试剂比例优化转染效率,保证细胞密度大于90%,DNA(μg): Hieff TransTM(μL)比值在1:0.5-1:5。 7) 本产品仅作科研用 … WebHieff Trans™ in vitro siRNA/miRNA Transfection Reagent is a PEI transfection reagent developed for the transfection of siRNA into mammalian cells. Suitable for transfection of …
WebHieff Trans™ PEI Transfection Reagent-GMP was used to transfect the suspension cell HEK293F cell line and adherent cell HEK293T cell line in a 6-well format. AAV-GFP …
Web13 de abr. de 2024 · 产品简介. Hieff Trans™脂质体核酸转染试剂是一种多用途的脂质体转染试剂，适用于DNA、RNA和寡核苷酸的转染，对大多数真核细胞具有很高的转染效率。. 其独特的配方使其可直接加入培养基中，血清的存在不会影响转染效率，这样可以减少去除血清对细胞的损伤 ... flaming mountain of lagourWebHieff Trans™ Liposomal Transfection Reagent is a versatile liposome transfection reagent, suitable for DNA, RNA, and oligonucleotide transfection, with high transfection efficiency … flamingo admin commands lavaWebHieff Trans™ universal transfection reagent is a multi-purpose, convenient and efficient liposome transfection reagent developed based on the latest nanotechnology. It is suitable for the transfection of DNA, RNA and oligonucleotides, including primary cells, Difficult-to-transfect cells, including neurons, have high transfection efficiency. flamingoair.comWebHieff Trans ® Liposomal Transfection Reagent 脂质体核酸转染试剂. Hieff Trans ® 脂质体核酸转染试剂是一种多用途的脂质体转染试剂，适用于 DNA 、 RNA 和寡核苷酸的转染，对大多数真核细胞具有很高的转染效率。 其独特的配方使其可直接加入培养基中，血清的存在不会影响转染效率，这样可以减少去除血清对 ... can pregnant women have a mammogramWebHieff TransTM complex was added to each well of the cell culture plate, followed by in-complete DMEM-H medium to a total volume of 2 mL. The culture plate was then gently shaken and mixed. After 6 h of culture at 37 °C in a 5% CO2 incubator, the growth medium was replaced. Further, after 48 h of culture, RNA was extracted, and the expression ... flamingo-ahornWeb5 de jan. de 2024 · The cells were seeded in 24-well plates and transfected using Hieff Trans Liposomal Transfection Reagent (Yeasen, China) according to the manufacturer’s instructions. After 72 h, the cells were collected and used for genotyping by TIDE 45 and EditR. 46 All target sites and primers used for genotyping are listed in Tables S4 and S5. … flamingo afghan crochet patternWeb>>>Hieff Trans转染试剂产品特点 高效性： 可对大多数真核细胞进行转染，并且对于常见细胞系转染效率达90%以上； 低毒性：转染细胞形态良好并表达大量的转染基因蛋白； 操作简便：可以直接将脂质体复合物加入到 … flamingo air from nassau